AroCell TK 210 ELISA for determination of TK1 protein: age-related reference ranges and comparison with other TK1 assays

Biotechniques. 2020 Jun;68(6):334-341. doi: 10.2144/btn-2019-0148. Epub 2020 Apr 27.

Abstract

Thymidine kinase 1 (TK1) is an enzyme involved in DNA precursor synthesis that has been used as a biomarker for prognosis and monitoring of different malignancies. In this study, we compared two immunoassays for measuring TK1 protein concentrations: the TK 210 ELISA (AroCell AB) and TK1 ELISA from Abcam. Overall, the TK 210 ELISA showed higher sensitivity than the Abcam TK1 ELISA for differentiating hematological malignancies (sensitivity of 0.77 vs 0.45) as well as for distinguishing sera of patients with solid tumors from those of apparently healthy individuals (0.61 vs 0.20). There was no significant difference in the TK1 protein levels determined with the TK 210 ELISA between different age groups from apparently healthy individuals. These results strongly indicate that the AroCell TK 210 ELISA is accurate and sensitive enough to be a valuable tool in cancer management.

Keywords: AroCell TK 210 ELISA; anti-human TK1 antibodies; hematological malignancies; serum thymidine kinase 1 (STK1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Biomarkers, Tumor / blood*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Female
  • Hematologic Neoplasms / blood*
  • Humans
  • Male
  • Middle Aged
  • Reference Values
  • Thymidine Kinase / blood
  • Thymidine Kinase / isolation & purification*

Substances

  • Biomarkers, Tumor
  • Thymidine Kinase
  • thymidine kinase 1